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1993-10-28
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DOCUMENTS 1 TO 7 PAGE = 1 OF 13
CAUL/ISI09 DOCUMENT= 1 OF 7
PUBDATE = 9309 INDATE = 930922
RT I
UI LU957-0021
TI KINETIC PCR ANALYSIS - REAL TIME MONITORING OF DNA AMPLIFICATION
REACTIONS
AU HIGUCHI R (Reprint). FOCKLER C. DOLLINGER G. WATSON R.
AD ROCHE MOLEC SYST INC, 1145 ATLANTIC AVE, ALAMEDA, CA, 94501 USA
(Reprint). CHIRON CORP, EMERYVILLE, CA, 94608 USA
SO BIO-TECHNOLOGY, 1993 SEP, v11, n9 p.1026-1030
AB We describe a simple, quantitative assay for any amplifiable DNA
sequence that uses a video camera to monitor multiple polymerase
chain reactions (PCRs) simultaneously over the course of
thermocycling. The video camera detects the accumulation of
double-stranded DNA (dsDNA) in each PCR using the increase in the
fluorescence of ethidium bromide (EtBr) that results from its binding
duplex DNA. The kinetics of fluorescence accumulation during
thermocycling are directly related to the starting number of DNA
copies. The fewer cycles necessary to produce a detectable
fluorescence, the greater the number of target sequences. Results
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DOCUMENTS 1 TO 7 PAGE = 2 OF 13
obtained with this approach indicate that a kinetic approach to PCR
analysis can quantitate DNA sensitively, selectively and over a large
dynamic range. This approach also provides a means of determining the
effect of different reaction conditions on the efficacy of the
amplification and so can provide insight into fundamental PCR
processes.
KP POLYMERASE CHAIN REACTION. ENZYMATIC AMPLIFICATION. SEQUENCES.
INVITRO
JS BIOTECHNOLOGY & APPLIED MICROBIOLOGY
RE 18 REFS
LA ENGLISH
DO ARTICLE
IS 0733-222X
TC For table of contents see UI LU957-0000
AA Y
GA LU957
CAUL/ISI09 DOCUMENT= 2 OF 7
PUBDATE = 9309 INDATE = 930922
RT I
UI LU957-0024
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DOCUMENTS 1 TO 7 PAGE = 3 OF 13
TI A REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION ASSAY FOR THE
DETECTION AND QUANTITATION OF MURINE RETROVIRUSES
AU IRVING J M. CHANG L W S. CASTILLO F J (Reprint).
AD XOMA CORP, 2910 7TH ST, BERKELEY, CA, 94710 USA (Reprint). XOMA
CORP, 2910 7TH ST, BERKELEY, CA, 94710 USA
SO BIO-TECHNOLOGY, 1993 SEP, v11, n9 p.1042-1046
AB Specific hybridization primers for the PCR assay were developed to
detect the presence of the ecotropic, xenotropic, and mink cell
focus-forming classes of murine leukemia viruses (MuLVs) in samples
derived from cultured cells and cell-free supernatants. The primers,
which were tested against reference viruses from all three classes
and two subclasses and accurately identified each class present, were
used to characterize the endogenous expression of MuLV-related
sequences in a number of murine and mink cell lines. Two
murine/murine hybridomas were shown to contain expressed retroviral
sequences from all three classes. The murine cell lines SC-1, Balb/c
3T3, and NIH 3T3, were found to constitutively express sequences from
many of the MuLV classes. These MuLV-related sequences were not
expressed in the Mus dunni or mink lung cell lines. When these
primers were used in a quantitative PCR assay to determine the
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DOCUMENTS 1 TO 7 PAGE = 4 OF 13
retroviral content of hybridoma supernatants, the values were less
variable than those obtained by transmission electron microscopy
(TEM). This assay can be adapted to detect and quantitate any viral
contaminant in cell culture supernatants, ascites fluids, process
validation samples, and final products.
KP LEUKEMIA VIRUSES. SEQUENCES. PROBES. MICE. RNA
JS BIOTECHNOLOGY & APPLIED MICROBIOLOGY
RE 34 REFS
LA ENGLISH
DO ARTICLE
IS 0733-222X
TC For table of contents see UI LU957-0000
AA Y
GA LU957
CAUL/ISI08 DOCUMENT= 3 OF 7
PUBDATE = 9300 INDATE = 930814
RT I
UI LB968-0037
TI APPLICATION OF THE POLYMERASE CHAIN REACTION TECHNIQUE TO THE
DETECTION OF PATHOGENS IN WATER
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DOCUMENTS 1 TO 7 PAGE = 5 OF 13
AU TORANZOS G A (Reprint). ALVAREZ A J. DVORSKY E A.
AD UNIV PUERTO RICO, DEPT BIOL, RIO PIEDRAS, PR, 00931 USA (Reprint)
SO WATER SCIENCE AND TECHNOLOGY, 1993 v27, n3-4 p.207-210
AB Enteric pathogens may be present in fecally contaminated waters at
extremely low concentrations. In addition, these pathogens may be
injured when exposed to the environment and may not be able to grow
in laboratory culture media or such media may simply not exist for
their progagation in the laboratory. It is paramount thus to use
techniques which do not depend on culture techniques for the
detection of these pathogens and that allow for the detection of
single-cell concentrations. The polymerase chain reaction (PCR)
technique has been shown to be an excellent and sensitive means of
detecting pathogens in waters. Membrane filtration has been combined
with PCR and DNA hybridization techniques to be able to detect the
DNA equivalent of one single cell in large volumes of water. In
addition, this combination of methods allows for the amplification of
different target genes that may be present in the sample, since the
membrane can be subjected to repeated amplification reactions under
different conditions. A Most Probable Number PCR was developed which
allows for the quantification of gene copy number and thus permits
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DOCUMENTS 1 TO 7 PAGE = 6 OF 13
extrapolation to estimate the number of bacterial cells in the
original sample.
AK MPN PCR. ENTERIC PATHOGENS. WATER MICROBIOLOGY. QUANTITATIVE PCR
KP DNA
JS WATER RESOURCES. ENVIRONMENTAL SCIENCES. ENGINEERING, CIVIL
RE 14 REFS
LA ENGLISH
DO ARTICLE
IS 0273-1223
TC For table of contents see UI LB968-0000
AA Y
GA LB968
CAUL/ISI08 DOCUMENT= 4 OF 7
PUBDATE = 9307 INDATE = 930814
RT I
UI LM932-0002
TI AN ELECTROCHEMILUMINESCENCE BASED DETECTION SYSTEM FOR QUANTITATIVE
PCR
AU ANDERSON M S (Reprint). DICESARE J L. KATZ E D.
AD PERKIN ELMER CORP, INSTRUMENT MARKETING, 761 MAIN AVE, NORWALK, CT,
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DOCUMENTS 1 TO 7 PAGE = 7 OF 13
06859 USA (Reprint)
SO AMERICAN BIOTECHNOLOGY LABORATORY, 1993 JUL, v11, n8 p.10-10
JS BIOTECHNOLOGY & APPLIED MICROBIOLOGY
RE NO REFS
LA ENGLISH
DO ARTICLE
IS 0749-3223
TC For table of contents see UI LM932-0000
AA N
GA LM932
CAUL/ISI08 DOCUMENT= 5 OF 7
PUBDATE = 9302 INDATE = 930812
RT I
UI KP837-0008
TI USING RANDOMLY AMPLIFIED POLYMORPHIC DNA FOR EVALUATING GENETIC
RELATIONSHIPS AMONG PAPAYA CULTIVARS
AU STILES J I (Reprint). LEMME C. SONDUR S. MORSHIDI M B.
MANSHARDT R.
AD UNIV HAWAII, COLL TROP AGR & HUMAN RESOURCES, DEPT PLANT MOLEC
PHYSIOL, HONOLULU, HI, 96822 USA (Reprint). UNIV HAWAII, COLL TROP
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DOCUMENTS 1 TO 7 PAGE = 8 OF 13
AGR & HUMAN RESOURCES, DEPT HORT, HONOLULU, HI, 96822 USA
SO THEORETICAL AND APPLIED GENETICS, 1993 FEB, v85, n6-7 p.697-701
AB We have applied the recently developed technique of random
amplification of polymorphic DNA (RAPD) to the analysis of the
relationships among ten cultivars of papaya (Carica papaya L.).
Eleven ten-base synthetic oligonucleotides were chosen that gave
multiple PCR amplification products using papaya DNA as template.
These 11 primers amplified a total of 102 distinct fragments.
Cultivars were scored for presence or absence of RAPD fragments and
grouped by cluster analysis using simple matching coefficients of
similarity. A dendrogram of the ten cultivars was constructed. Of the
ten cultivars seven were of the Hawaiian type, and all of these
grouped to one branch of the tree. Divisions within the Hawaiian,
branch were mostly consistent with the known genetic background of
these cultivars. Three non-Hawaiian, cultivars were also analyzed.
The minimum similarity detected was 0.7 suggesting that the
domesticated papaya germ plasm is quite narrow. Our results show that
RAPD technology is a rapid, precise and sensitive technique for
genomic analysis.
AK CARICA PAPAYA L. POLYMERASE CHAIN REACTION. GENETIC POLYMORPHISM.
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DOCUMENTS 1 TO 7 PAGE = 9 OF 13
PHYLOGENETIC RELATIONSHIP
KP QUANTITATIVE TRAITS. MENDELIAN FACTORS. PCR. PRIMERS
JS GENETICS & HEREDITY
RE 16 REFS
LA ENGLISH
DO ARTICLE
IS 0040-5752
TC For table of contents see UI KP837-0000
AA Y
GA KP837
CAUL/ISI08 DOCUMENT= 6 OF 7
PUBDATE = 9303 INDATE = 930812
RT I
UI KT810-0036
TI HIGH LEVELS OF HIV 1 IN PLASMA DURING ALL STAGES OF INFECTION
DETERMINED BY COMPETITIVE PCR
AU PIATAK M. SAAG M S. YANG L C. CLARK S J. KAPPES J C. LUK K C.
HAHN B H. SHAW G M. LIFSON J D (Reprint).
AD GENELABS TECHNOL INC, DIV HIV & EXPLORATORY RES, REDWOOD CITY, CA,
94063 USA (Reprint). GENELABS TECHNOL INC, DIV HIV & EXPLORATORY
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DOCUMENTS 1 TO 7 PAGE = 10 OF 13
RES, REDWOOD CITY, CA, 94063 USA. UNIV ALABAMA, DEPT MED, DIV
HEMATOL ONCOL, BIRMINGHAM, AL, 35294 USA. UNIV ALABAMA, DEPT MED,
DIV INFECT DIS, BIRMINGHAM, AL, 35294 USA
SO SCIENCE, 1993 MAR 19, v259, n5102 p.1749-1754
AB Quantitative competitive polymerase chain reaction (QC-PCR) methods
were used to quantify virion-associated human immunodeficiency virus
type-1 (HIV-1) RNA in plasma from 66 patients with Centers for
Disease Control stage I to IVC1 infection. HIV-1 RNA, ranging from
100 to nearly 22,000,000 copies per milliliter of plasma
(corresponding to 50 to 11,000,000 virions per milliliter), was
readily quantified in all subjects, was significantly associated with
disease stage and CD4+ T cell counts, and decreased by as much as
235-fold with resolution of primary infection or institution of
antiretroviral therapy. Plasma virus levels determined by QC-PCR
correlated with, but exceeded by an average of 60,000-fold, virus
titers measured by endpoint dilution culture. Quantitation of HIV-1
in plasma by QC-PCR may be useful in assessing the efficacy of
antiretroviral agents, especially in early stage disease when
conventional viral markers are often negative.
KP HUMAN IMMUNODEFICIENCY VIRUS. POLYMERASE CHAIN REACTION. T CELL
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DOCUMENTS 1 TO 7 PAGE = 11 OF 13
RECEPTOR. HTLV III. LEUKEMIA VIRUS. SAN FRANCISCO. MESSENGER
RNA. AIDS. DISEASE. QUANTITATION
JS MULTIDISCIPLINARY SCIENCES
RE 71 REFS
LA ENGLISH
DO ARTICLE
IS 0036-8075
TC For table of contents see UI KT810-0000
AA Y
GA KT810
CAUL/ISI08 DOCUMENT= 7 OF 7
PUBDATE = 9300 INDATE = 930812
RT I
UI KW281-0002
TI REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION - AN OVERVIEW OF THE
TECHNIQUE AND ITS APPLICATIONS
AU OHAN N W (Reprint). HEIKKILA J J.
AD UNIV WATERLOO, DEPT BIOL, WATERLOO N2L 3G1, ONTARIO, CANADA (Reprint)
SO BIOTECHNOLOGY ADVANCES, 1993 v11, n1 p.13-29
AB The polymerase chain reaction (PCR) has galvanized molecular
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DOCUMENTS 1 TO 7 PAGE = 12 OF 13
biologists by virtue of its ability to provide them with large
quantities of any desired fragment (up to 11kb) of DNA. This power
combined with its flexibility has also inspired many useful
applications, including new methods of DNA sequencing, cloning and
mutagenesis. One logical variation of PCR has been its application to
the detection and analysis of messenger RNA by the addition of a
reverse transcription step prior to performing PCR. Due to the
exquisite sensitivity of PCR, reverse transcription-PCR (RT-PCR) has
been used to characterize mRNAs previously undetectable by
established methods of RNA analysis such as Northern hybridization
and RNase protection assays. Furthermore, its capacity as a method of
quantitative analysis is currently being developed. RT-PCR has also
been used to diagnose the presence of certain diseases. Recently,
RT-PCR has been employed to identify and isolate genes that are
differentially expressed in different cells or environmental
conditions.
AK REVERSE TRANSCRIPTION. POLYMERASE CHAIN REACTION (PCR). RT PCR.
MESSENGER RNA. CDNA. PRIMER DESIGN. CONTAMINATION. QUANTITATION
KP MESSENGER RNA. DNA AMPLIFICATION. RIBONUCLEIC ACID. GENERAL
METHOD. PCR. INVITRO. HYBRIDIZATION. CONTAMINATION.
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DOCUMENTS 1 TO 7 PAGE = 13 OF 13
QUANTITATION. TEMPERATURE
JS BIOTECHNOLOGY & APPLIED MICROBIOLOGY
RE 53 REFS
LA ENGLISH
DO REVIEW. NONARTICLE
IS 0734-9750
TC For table of contents see UI KW281-0000
AA Y
GA KW281
R0601 * END OF D